immune responses against a new hiv-1 p24-gp41/pcaggs-il-12 dna vaccine in balb/c mice
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abstract
background: development of an effective vaccine is highly needed in order to restrict the aids pandemic. dna vaccines initiate both arms of immunity without the potential of causing disease. hiv-1 p24 and gp41 (gag and env) proteins play important roles in viral pathogenesis and are effective candidates for immune induction and vaccine design. objective: in this study, new dna vaccine candidates constructed from hiv-1 fused p24- gp41 or gp41 alone were evaluated in balb/c mice for induction of cellular and humoral immune responses. methods: recombinant plasmids, pcdna3.1/hygro expression vector containing immunogenic sequences of fused p24-gp41 or gp41alone were produced. dendrosome used as a system for carrying vectors in laboratory animals, and an il-12 containing vector (pcaggs-il-12) was co-immunized with the p24-gp41 vector as a genetic adjuvant. induction of effective immune responses against the designed vectors as dna vaccine candidates in balb/c mice was evaluated. levels of total antibodies, igg isotypes (igg2a and igg1); ifn-γ and il-4 were measured by elisa. mtt assay was used to evaluate lymphoproliferation. results: the results confirmed that the immunogenic epitopes of both p24 and gp41 genes are highly effective inducers of immune responses, and administration of fused p24-gp41 alone or along with il-12 resulted in further enhancement of immune responses. group 4 that received fused fragments (p24-gp41) along with an il-12 expressing vector demonstrated a significantly higher stimulation index (si) and ifn- production (p
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Journal title:
iranian journal of immunologyجلد ۹، شماره ۲، صفحات ۸۶-۹۷
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